Genetic Engineering Publications - GEG Tech top picks

In this article, the scientists  present an approach, called leveraging endogenous ADAR for programmable editing of RNA (LEAPER), that employs short engineered ADAR-recruiting RNAs (arRNAs) to recruit native ADAR1 or ADAR2 enzymes to change a specific adenosine to inosine. They pave the way for a single-molecule system, LEAPER, enabling precise, efficient RNA editing with broad applicability for therapy and basic research.

Here the authors demonstrate that the class 2 type VI RNA-guided RNA-targeting CRISPR–Cas effector Cas13a (previously known as C2c2) can be engineered for mammalian cell RNA knockdown and binding. Their results establish CRISPR–Cas13a as a flexible platform for studying RNA in mammalian cells and therapeutic development.